[ トップページ | メンバー | 研究内容 | 研究業績 | リンク | English ]

iwadate @ yamaguchi-u.ac.jp
Faculty of Science
Yamaguchi University
Yamaguchi 753-8512, Japan


Lab Members
    Staff
      IWADATE Yoshiaki
      OKIMURA Chika

    Graduate and Undergraduate Students


Research Theme

We are interested in self-organization mechanisms in cell migrations, such as polarity in crawling cell migration and ciliary metachronal wave system and so on.

Crawling Cell Migration(keratocytes) Crawling Cell Migration(HL-60 cells)
taken by Okimura C taken by Okimura C

Ciliary Metachronal Wave (P. caudatum)
taken by Narematsu N


Approach and Techniques

Biophysical Approach.
Live cell imaging under microscopies; CLSM, TIRFM, Traction force Microscopy, ...
Mechatronics; lathe, CNC milling machine, ...
Electronics; OP Amp, CMOS, TTL, ...
Programing; LabVIEW, MATLAB, Quick BASIC, ...

CLSM
YOKOGAWA Spin Disk
Actin Filaments
in D. discoideum
under CLSM
Myosin II Filaments
in D. discoideum
under CLSM

Lathe Electronics Lab Made Cell
Stretching Device


Papers (2008-)
  • Danno, K., Nakamura, T., Okoso, N., Nakamura, N., Iguchi, K., Iwadate, Y., *Kenmotsu, T., Ikegawa, M., Uemoto, S., *Yoshikawa, K. (2018). Cracking pattern of tissue slices induced by external extension provides useful diagnostic information. Scientific Reports 8: 12167. doi: 10.1038/s41598-018-30662-9
  • Okimura, C., Taniguchi, A., Nonaka, S. and *Iwadate, Y. (2018). Rotation of stress fibers as a single wheel in migrating fish keratocytes. Scientific Reports 8: 10615. doi: 10.1038/s41598-018-28875-z
  • Okimura, C., Sakumura, Y., Shimabukuro, K. and *Iwadate, Y. (2018). Sensing of substratum rigidity and directional migration by fast-crawling cells. Physical Review E 97: 052401. doi: 10.1103/PhysRevE.97.052401
  • Okimura, C. and Iwadate, Y. (2017). Directional cell migration in response to repeated substratum stretching. Journal of the Physical Society of Japan 86: 101002. doi: 10.7566/JPSJ.86.101002
  • Okimura, C. and Iwadate, Y. (2016). Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes. Cell Adhesion & Migration. 10: 406-418. doi: 10.1080/19336918.2016.1170268
  • Okimura, C., Ueda, K., Sakumura, Y. and Iwadate, Y. (2016). Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching. Cell Adhesion & Migration. 10: 331-341. doi: 10.1080/19336918.2015.1129482
  • Adachi, K., Tokushige, M., Omata, K., Yamazaki, S. and Iwadate, Y. (2016). Kinetics of coloration in photochromic Tungsten(VI) Oxide/Silicon Oxycarbide/Silica hybrid xerogel: Insight into cation self-diffusion mechanisms. ACS Applied Materials & Interfaces. 8: 14019−14028. doi: 10.1021/acsami.6b04115
  • Sonoda, A., Okimura, C. and Iwadate, Y. (2016). Shape and area of keratocytes are related to the distribution and magnitude of their traction forces. Cell Structure and Function 41: 33-43. doi: 10.1247/csf.15008
  • Nakata, T., Okimura, C., Mizuno, T. and Iwadate, Y. (2016). The role of stress fibers in the shape determination mechanism of fish keratocytes. Biophysical Journal 110: 481-492. doi: 10.1016/j.bpj.2015.12.014
  • Narematsu, N., Quek, R., Chiam, K.-H. and Iwadate, Y. (2015). Ciliary metachronal wave propagation on the compliant surface of Paramecium cells. Cytoskeleton 72: 633-646. doi: 10.1002/cm.21266
  • Nakashima, H., Okimura, C. and Iwadate, Y. (2015). The molecular dynamics of crawling migration in microtubule-disrupted keratocytes. Biophysics and Physicobiology 12: 21-29. doi: 10.2142/biophysico.12.0_21
  • Tsugiyama, H., Okimura, C., Mizuno, T. and Iwadate, Y. (2013). Electroporation of adherent cells with low sample volumes on a microscope stage. Journal of Experimental Biology 216: 3591-3598. doi: 10.1242/jeb.089870
  • Iwadate, Y., Okimura, C., Sato, K., Nakashima, Y., Tsujioka, M. and Minami, K. (2013). Myosin-II-mediated directional migration of Dictyostelium cells in response to cyclic stretching of substratum. Biophysical Journal 104: 748-758. doi: 10.1016/j.bpj.2013.01.005
  • Uyeda, Q.P.T., Iwadate, Y., Umeki, N., Nagasaki, A. and Yumura, S. (2011). Stretching actin filaments within cells enhances their affinity for the myosin II motor domain. PLoS ONE 6: e26200. doi: 10.1016/j.bpj.2013.01.005
  • Iwadate, Y. and Yumura, S. (2009). Cyclic stretch of the substratum using a shape-memory alloy induces directional migration in Dictyostelium cells. Biotechniques 47: 757-767. doi: 10.2144/000113217
  • Pramanik, Md. K., Iijima, M., Iwadate, Y. and Yumura, S. (2009). PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells. Genes to Cells 14: 821-834. doi: 10.1111/j.1365-2443.2009.01312.x
  • Iwadate, Y. and Nakaoka, Y. (2008). Calcium regulates independently ciliary beat and cell contraction in Paramecium cells. Cell Calcium 44: 169-179. doi: 10.1016/j.ceca.2007.11.006
  • Iwadate, Y. and Yumura, S. (2008). Actin-based propulsive force and myosin II-based contractile force in a migrating Dictyostelium cells. Journal of Cell Science 121: 1314-1324. doi: 10.1242/jcs.021576
  • Iwadate, Y. and Yumura, S. (2008). Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies. Biotechniques 44: 739-750. doi: 10.2144/000112752


Faculty of Science, Yamaguchi University
© 2008 Iwadate Y