Lab Members
Staff
IWADATE Yoshiaki 
OKIMURA Chika
Graduate and Undergraduate Students
Research Theme
We are interested in self-organization mechanisms in cell migrations, such as polarity in crawling cell migration and ciliary metachronal wave system and so on.
| Crawling Cell Migration（keratocytes） |
Crawling Cell Migration（HL-60 cells） |
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| taken by Okimura C |
taken by Okimura C |
| Ciliary Metachronal Wave (P. caudatum) |
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| taken by Narematsu N |
Approach and Techniques
Biophysical Approach.
Live cell imaging under microscopies; CLSM, TIRFM, Traction force Microscopy, ...
Mechatronics; lathe, CNC milling machine, ...
Electronics; OP Amp, CMOS, TTL, ...
Programing; LabVIEW, MATLAB, Quick BASIC, ...
CLSM
YOKOGAWA Spin Disk |
Actin Filaments
in D. discoideum
under CLSM |
Myosin II Filaments
in D. discoideum
under CLSM |
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| Lathe |
Electronics |
Lab Made Cell
Stretching Device |
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Papers (2008-)
- Fujikawa R., Okimura C, Kozawa S, Ikeda K, Inagaki N, Iwadate Y, Sakumura Y. (2023). Bayesian traction force estimation using cell boundary-dependent force priors. Biophys. J. 122: 4542-4554.doi: 10.1016/j.bpj.2023.10.032
- Okimura, C., Akiyama, S., Nishigami, Y., Zaitsu, R., Sakurai, T. and *Iwadate Y. (2023). Linear contraction of stress fibers generates cell body rotation. bioRxiv. doi: 10.1101/2023.01.03.522661
- Okimura, C., Iwanaga, M., Sakurai, T., Ueno, T., Urano, Y. and *Iwadate Y. (2022). Leading edge elongation by follower cell interruption in advancing epithelial cell sheets. PNAS 119: e2119903119. doi: 10.1073/pnas.2119903119
- Takagi, T., Ueno, T., Ikawa, K., Asanuma, D., Nomura, Y., Uno, S., Komatsu, T., Kamiya, M., Hanaoka, K., Okimura, C., Iwadate, Y., Hirose, K., Nagano, T., Sugimura, K. and Urano, Y. (2021). Discovery of an F-actin-binding small molecule serving as a fluorescent probe and a scaffold for functional probes. Science Advances 7(47) eabg8585. doi: 10.1126/sciadv.abg8585
- Imoto, D., Saito, N., Nakajima, A., Honda, G., Ishida, M., Sugita, T., Ishihara, S., Katagiri, K., Okimura, C., Iwadate, Y. and Sawai, S. (2021). Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space. PLoS Computational Biology 17(8) e1009237. doi: 10.1371/journal.pcbi.1009237
- Tsujioka, M., Uyeda, T.Q.P., Iwadate, Y., Patel, H., Shibata, K., Yumoto, T. and Yonemura, S. (2019). Actin-binding domains mediate the distinct distribution of two Dictyostelium Talins through different affinities to specific subsets of actin filaments during directed cell migration. PloS One 14: e0214736. doi: 10.1371/journal.pone.0214736
- Danno, K., Nakamura, T., Okoso, N., Nakamura, N., Iguchi, K., Iwadate, Y., Kenmotsu, T., Ikegawa, M., Uemoto, S., Yoshikawa, K. (2018). Cracking pattern of tissue slices induced by external extension provides useful diagnostic information. Scientific Reports 8: 12167. doi: 10.1038/s41598-018-30662-9
- Okimura, C., Taniguchi, A., Nonaka, S. and Iwadate, Y. (2018). Rotation of stress fibers as a single wheel in migrating fish keratocytes. Scientific Reports 8: 10615. doi: 10.1038/s41598-018-28875-z
- Okimura, C., Sakumura, Y., Shimabukuro, K. and *Iwadate, Y. (2018). Sensing of substratum rigidity and directional migration by fast-crawling cells. Physical Review E 97: 052401. doi: 10.1103/PhysRevE.97.052401
- Okimura, C. and Iwadate, Y. (2017). Directional cell migration in response to repeated substratum stretching. Journal of the Physical Society of Japan 86: 101002. doi: 10.7566/JPSJ.86.101002
- Okimura, C. and Iwadate, Y. (2016). Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes. Cell Adhesion & Migration 10: 406-418. doi: 10.1080/19336918.2016.1170268
- Okimura, C., Ueda, K., Sakumura, Y. and Iwadate, Y. (2016). Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching. Cell Adhesion & Migration 10: 331-341. doi: 10.1080/19336918.2015.1129482
- Adachi, K., Tokushige, M., Omata, K., Yamazaki, S. and Iwadate, Y. (2016). Kinetics of coloration in photochromic Tungsten(VI) Oxide/Silicon Oxycarbide/Silica hybrid xerogel: Insight into cation self-diffusion mechanisms. ACS Applied Materials & Interfaces 8: 14019−14028. doi: 10.1021/acsami.6b04115
- Sonoda, A., Okimura, C. and Iwadate, Y. (2016). Shape and area of keratocytes are related to the distribution and magnitude of their traction forces. Cell Structure and Function 41: 33-43. doi: 10.1247/csf.15008
- Nakata, T., Okimura, C., Mizuno, T. and Iwadate, Y. (2016). The role of stress fibers in the shape determination mechanism of fish keratocytes. Biophysical Journal 110: 481-492. doi: 10.1016/j.bpj.2015.12.014
- Narematsu, N., Quek, R., Chiam, K.-H. and Iwadate, Y. (2015). Ciliary metachronal wave propagation on the compliant surface of Paramecium cells. Cytoskeleton 72: 633-646. doi: 10.1002/cm.21266
- Nakashima, H., Okimura, C. and Iwadate, Y. (2015). The molecular dynamics of crawling migration in microtubule-disrupted keratocytes. Biophysics and Physicobiology 12: 21-29. doi: 10.2142/biophysico.12.0_21
- Tsugiyama, H., Okimura, C., Mizuno, T. and Iwadate, Y. (2013). Electroporation of adherent cells with low sample volumes on a microscope stage. Journal of Experimental Biology 216: 3591-3598. doi: 10.1242/jeb.089870
- Iwadate, Y., Okimura, C., Sato, K., Nakashima, Y., Tsujioka, M. and Minami, K. (2013). Myosin-II-mediated directional migration of Dictyostelium cells in response to cyclic stretching of substratum. Biophysical Journal 104: 748-758. doi: 10.1016/j.bpj.2013.01.005
- Uyeda, Q.P.T., Iwadate, Y., Umeki, N., Nagasaki, A. and Yumura, S. (2011). Stretching actin filaments within cells enhances their affinity for the myosin II motor domain. PLoS ONE 6: e26200. doi: 10.1016/j.bpj.2013.01.005
- Iwadate, Y. and Yumura, S. (2009). Cyclic stretch of the substratum using a shape-memory alloy induces directional migration in Dictyostelium cells. Biotechniques 47: 757-767. doi: 10.2144/000113217
- Pramanik, Md. K., Iijima, M., Iwadate, Y. and Yumura, S. (2009). PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells. Genes to Cells 14: 821-834. doi: 10.1111/j.1365-2443.2009.01312.x
- Iwadate, Y. and Nakaoka, Y. (2008). Calcium regulates independently ciliary beat and cell contraction in Paramecium cells. Cell Calcium 44: 169-179. doi: 10.1016/j.ceca.2007.11.006
- Iwadate, Y. and Yumura, S. (2008). Actin-based propulsive force and myosin II-based contractile force in a migrating Dictyostelium cells. Journal of Cell Science 121: 1314-1324. doi: 10.1242/jcs.021576
- Iwadate, Y. and Yumura, S. (2008). Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies. Biotechniques 44: 739-750. doi: 10.2144/000112752
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